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TBPL2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TBPL2 Antibody (N-term) AP12171A
    TBPL2 Antibody (N-term) (Cat. #AP12171a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TBPL2 antibody detected the TBPL2 protein (arrow).
  • IHC-P - TBPL2 Antibody (N-term) AP12171A
    TBPL2 Antibody (N-term) (Cat. #AP12171a)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBPL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - TBPL2 Antibody (N-term) AP12171A
    Fluorescent confocal image of Hela cell stained with TBPL2 Antibody (N-term)(Cat#AP12171a).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBPL2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBPL2 immunoreactivity is localized to Nucleus and Cytoplasm significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6SJ96
Other Accession NP_950248.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 41524 Da
Antigen Region 12-39 aa
Additional Information
Gene ID 387332
Other Names TATA box-binding protein-like protein 2, TBP-like protein 2, TATA box-binding protein-related factor 3, TBP-related factor 3, TBPL2 {ECO:0000312|EMBL:AAI171861, ECO:0000312|HGNC:HGNC:19841}
Target/Specificity This TBPL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human TBPL2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBPL2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TBPL2 {ECO:0000312|EMBL:AAI17186.1, ECO:0000312|HGNC:HGNC:19841}
Function Transcription factor required in complex with TAF3 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process (By similarity).
Cellular Location Cytoplasm. Nucleus. Note=Present in the cytoplasm during cytokenesis.
Tissue Location Ubiquitously expressed in all tissues examined with highest levels in heart, lung, ovary, spleen and testes
Citations (0)

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TBPL2 is a transcription factor required in complex with TAF3 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process (By similarity).


Rose, J. Phd, et al. Mol. Med. (2010) In press :
Deato, M.D., et al. Genes Dev. 21(17):2137-2149(2007)
Di Pietro, C., et al. DNA Cell Biol. 26(6):369-385(2007)
Persengiev, S.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(25):14887-14891(2003)

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$ 295.00
$ 99.00
Cat# AP12171A
(40 western blots)
Availability: In Stock
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