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GDF9 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GDF9 Antibody (N-term) AP12182a
    GDF9 Antibody (N-term) (Cat. #AP12182a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GDF9 antibody detected the GDF9 protein (arrow).
  • IHC-P - GDF9 Antibody (N-term) AP12182a
    GDF9 Antibody (N-term) (Cat. #AP12182a)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GDF9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60383
Other Accession NP_005251.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51444 Da
Antigen Region 80-109 aa
Additional Information
Gene ID 2661
Other Names Growth/differentiation factor 9, GDF-9, GDF9
Target/Specificity This GDF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human GDF9.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGDF9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GDF9
Function Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
Cellular Location Secreted.
Tissue Location Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level)
Research Areas
Citations (0)

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Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. Growth differentiation factor-9 (GDF9) is expressed in oocytes and is thought to be required for ovarian folliculogenesis. GDF9 is a member of the transforming growth factor-beta superfamily.


Bokobza, S.M., et al. J. Cell. Physiol. 225(2):529-536(2010)
Wang, T.T., et al. Fertil. Steril. 94(6):2490-2492(2010)
Shi, F.T., et al. J. Clin. Endocrinol. Metab. 95 (10), E172-E180 (2010) :
Sproul, K., et al. BJOG 117(6):756-760(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)

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Cat# AP12182a
(40 western blots)
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