|Application ||WB, IHC-P, IF, E|
|Other Accession||P0C643, Q9QUG9, A6N9I4, NP_001092141.1, NP_722541.1|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||69248 Da|
|Antigen Region||7-34 aa|
|Other Names||RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, CalDAG-GEFI, Cdc25-like protein, hCDC25L, F25B33 kinase-like protein, RASGRP2, CDC25L, MCG7|
|Target/Specificity||This RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human RASGRP2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RASGRP2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.|
|Cellular Location||Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell projection, ruffle membrane; Peripheral membrane protein. Note=Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons (By similarity)|
|Tissue Location||Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.
Nagamine, K., et al. Biol. Pharm. Bull. 33(7):1138-1142(2010)
Katagiri, K., et al. J. Biol. Chem. 279(12):11875-11881(2004)
Eto, K., et al. Proc. Natl. Acad. Sci. U.S.A. 99(20):12819-12824(2002)
Toki, S., et al. J. Comp. Neurol. 437(4):398-407(2001)
Guo, F.F., et al. J. Biol. Chem. 276(27):25568-25581(2001)
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