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TCRB Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TCRB Antibody (Center) AP12193c
    Anti-TCRB Antibody (Center) at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TCRB Antibody (Center) AP12193c
    All lanes : Anti-TCRB Antibody (Center)(AP12193c) at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MOLT-4 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TCRB Antibody (Center) AP12193c
    Anti-TCRB Antibody (Center)(AP12193c) at 1:500 dilution + MDA-MB-231 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TCRB Antibody (Center) AP12193c
    Anti-TCRB Antibody (Center) at 1:1000 dilution + human spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size :15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TCRB Antibody (Center) AP12193c
    TCRB Antibody (Center) (Cat. #AP12193c) western blot analysis in MDA-MB231,HEK293,Ramos,NCI-H460 cell line lysates (35ug/lane).This demonstrates the TCRB antibody detected the TCRB protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P04435
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 14999 Da
Antigen Region 67-94 aa
Additional Information
Other Names T-cell receptor beta chain V region CTL-L17, TCRB
Target/Specificity This TCRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-94 amino acids from the Central region of human TCRB.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTCRB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCRB
Research Areas
Citations (0)

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Background

The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.

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$ 295.00
$ 99.00
Cat# AP12193c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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