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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PRAMEF8 Antibody (C-term)   

PRAMEF8 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PRAMEF8 Antibody (C-term) AP12211b
    PRAMEF8 Antibody (C-term) (Cat. #AP12211b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRAMEF8 antibody detected the PRAMEF8 protein (arrow).
  • IHC-P - PRAMEF8 Antibody (C-term) AP12211b
    PRAMEF8 Antibody (C-term) (Cat. #AP12211b)immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRAMEF8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - PRAMEF8 Antibody (C-term) AP12211b
    PRAMEF8 Antibody (C-term) (Cat. #AP12211b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5VWM4
Other Accession A6NMC2, O95522, NP_001012276.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 53655 Da
Antigen Region 419-447 aa
Additional Information
Gene ID 391002
Other Names PRAME family member 8, PRAMEF8
Target/Specificity This PRAMEF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-447 amino acids from the C-terminal region of human PRAMEF8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRAMEF8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PRAMEF24
Research Areas
Citations (0)

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Gregory, S.G., et al. Nature 441(7091):315-321(2006)

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Cat# AP12211b
(40 western blots)
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