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GPR45 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GPR45 Antibody (Center) AP12248c
    Anti-GPR45 Antibody (Center) at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GPR45 Antibody (Center) AP12248c
    All lanes : Anti-GPR45 Antibody (Center) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GPR45 Antibody (Center) AP12248c
    GPR45 Antibody (Center) (Cat. #AP12248c) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GPR45 antibody detected the GPR45 protein (arrow).
  • IHC-P - GPR45 Antibody (Center) AP12248c
    GPR45 Antibdy (Center) (Cat. #AP12248c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPR45 Antibdy (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y5Y3
Other Accession Q9EQQ4, NP_009158.3
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 41967 Da
Antigen Region 121-149 aa
Additional Information
Gene ID 11250
Other Names Probable G-protein coupled receptor 45, PSP24-1, PSP24-alpha, GPR45
Target/Specificity This GPR45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-149 amino acids from the Central region of human GPR45.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPR45 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPR45
Function Orphan receptor. May play a role in brain function.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Expressed in brain; detected in the basal forebrain, frontal cortex, and caudate, but not in thalamus, hippocampus, or putamen
Research Areas
Citations (0)

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This intronless gene encodes a member of the G protein-coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. This protein may function in the central nervous system.


Salonen, J.T., et al. Am. J. Hum. Genet. 81(2):338-345(2007) Wu, C., et al. Proteomics 7(11):1775-1785(2007) Hillier, L.W., et al. Nature 434(7034):724-731(2005) Kawasawa, Y., et al. Biochem. Biophys. Res. Commun. 276(3):957-964(2000) Kawasawa, Y., et al. Biochem. Biophys. Res. Commun. 276(3):952-956(2000)

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$ 295.00
$ 99.00
Cat# AP12248c
(40 western blots)
Availability: In Stock
Bulk Size
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