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SUMO2/3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - SUMO2/3 Antibody (C-term) AP1224a
    Fluorescent image of SH-SY5Y cells stained with SUMO2/3 Antibody (C-term) (Cat#AP1224a). AP1224a was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • IF - SUMO2/3 Antibody (C-term) AP1224a
    Fluorescent image of Hela cells stained with SUMO2/3 Antibody (C-term) (Cat#AP1224a). AP1224a was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • IF - SUMO2/3 Antibody (C-term) AP1224a
    Fluorescent image of U251 cells stained with SUMO2/3 Antibody(C-term) (Cat#AP1224a). AP1224a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - SUMO2/3 Antibody (C-term) AP1224a
    Western blot analysis of lysates from 293T, Hela, HL-60, Jurkat cell lines and rat liver tissue lysate (from left to right), using SUMO2/3 Antibody (Q65) (Cat. # AP1224a). AP1224a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - SUMO2/3 Antibody (C-term) AP1224a
    SUMO2/3 Antibody (C-term) (Cat.# AP1224a) western blot analysis in U251 cell lysate,mouse liver and rat liver tissue lysates (35ug/lane). This demonstrates that the SUMO2/3 antibody detected SUMO2/3 protein (arrow).
  • WB - SUMO2/3 Antibody (C-term) AP1224a
    SUMO2/3 Antibody (C-term) (Cat.# AP1224a) western blot analysis in Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).
  • WB - SUMO2/3 Antibody (C-term) AP1224a
    The anti-SUMO2/3 C-term Pab (Cat. #AP1224a) is used in Western blot to detect SUMO2/3 in HeLa cell lysate.
  • WB - SUMO2/3 Antibody (C-term) AP1224a
    Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (Q65) (Cat. #AP1224a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.
  • IHC-P - SUMO2/3 Antibody (C-term) AP1224a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P55854
Other Accession Q7SZ22, Q5XIF4, Q9Z172, Q6DI05, Q17QV3, P61959, P61958, P61957, Q2PFW2, P61956, Q6DHL4, Q6LDZ8, Q5ZJM9, P61955, Q6NV25, Q6GPW2, Q7ZTK7
Reactivity Human, Mouse
Predicted Rat, Zebrafish, Hamster, Monkey, Pig, Chicken, Xenopus, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 11637 Da
Antigen Region 49-81 aa
Additional Information
Gene ID 6612
Other Names Small ubiquitin-related modifier 3, SUMO-3, SMT3 homolog 1 {ECO:0000312|HGNC:HGNC:11124}, SUMO-2, Ubiquitin-like protein SMT3A, Smt3A, SUMO3 (HGNC:11124)
Target/Specificity This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-81 amino acids from the C-terminal region of human SUMO2/3.
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSUMO2/3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SUMO3 (HGNC:11124)
Function Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 (PubMed:11451954, PubMed:18538659, PubMed:21965678). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744).
Cellular Location Cytoplasm. Nucleus. Nucleus, PML body
Tissue Location Expressed predominantly in liver.
Research Areas
Citations ( 0 )

Background

SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Lapenta, V., et al., Genomics 40(2):362-366 (1997).

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$ 295.00
$ 99.00
Cat# AP1224a
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(40 western blots)
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