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NEDD8 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1226a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
NEDD8 Antibody (N-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q15843 |
| Other Accession | Q71UE8, Q4PLJ0, P29595, P61282 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat, Rabbit |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 9072 Da |
NEDD8 Antibody (N-term) - Additional info | |
| Gene ID 4738 | |
| Other Names NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; Ubiquitin-like protein Nedd8 | |
| Target/Specificity This NEDD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids from the N-terminal region of human NEDD8. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions NEDD8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
NEDD8 Antibody (N-term) - Protein Information | |
| Name NEDD8 | |
| Function Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins | |
| Cellular Location Nucleus. Note=Mainly nuclear. | |
| Tissue Location Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes | |
NEDD8 Antibody (N-term) - Related products
AP1226a: NEDD8 Antibody (N-term)
AP1227a: NEDD8 Antibody (C-term)
RI13543: NEDD8 predesign siRNA
DC09342: Human NEDD8 cDNA Clone
BP1226a: NEDD8 Antibody (N-term) Blocking Peptide
NEDD8 Antibody (N-term) - Application data
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Western blot analysis of NEDD8 polyclonal antibody (Cat. #AP1226a) in HeLa cell lysate. NEDD8 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
NEDD8 Antibody (N-term) - Research Areas
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BACKGROUND
NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
REFERENCES
Wu, K., et al., J. Biol. Chem. 278(31):28882-28891 (2003). Bohnsack, R.N., et al., J. Biol. Chem. 278(29):26823-26830 (2003). Fan, M., et al., Mol. Endocrinol. 17(3):356-365 (2003). Walden, H., et al., Nature 422(6929):330-334 (2003). Liu, J., et al., Mol. Cell 10(6):1511-1518 (2002).
