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FADS2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IF - FADS2 Antibody (N-term) AP12296A
    Fluorescent image of A549 cell stained with FADS2 Antibody (N-term)(Cat#AP12296a/SA110322AN).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.
  • WB - FADS2 Antibody (N-term) AP12296A
    FADS2 Antibody (N-term) (Cat. #AP12296a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).
  • IHC-P - FADS2 Antibody (N-term) AP12296A
    FADS2 Antibody (N-term) (Cat. #AP12296a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FADS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - FADS2 Antibody (N-term) AP12296A
    FADS2 Antibody (N-term) (Cat. #AP12296a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95864
Other Accession Q4R749, NP_004256.1
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52259 Da
Antigen Region 79-108 aa
Additional Information
Gene ID 9415
Other Names Fatty acid desaturase 2, 11419-, Delta(6) fatty acid desaturase, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2
Target/Specificity This FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the N-terminal region of human FADS2.
Dilution IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFADS2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FADS2
Function Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma- linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location Expressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index.
Research Areas
Citations ( 0 )


The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Mathias, R.A., et al. J. Lipid Res. 51(9):2766-2774(2010)
Lu, Y., et al. Am. J. Clin. Nutr. 92(1):258-265(2010)
Zabaneh, D., et al. PLoS ONE 5 (8), E11961 (2010) :
Steer, C.D., et al. PLoS ONE 5 (7), E11570 (2010) :

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$ 295.00
$ 99.00
Cat# AP12296A
(40 western blots)
Availability: In Stock
Bulk Size
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