MBOAT4 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | Q96T53 |
Other Accession | NP_001094386.1 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 49716 Da |
Antigen Region | 258-287 aa |
Gene ID | 619373 |
---|---|
Other Names | Ghrelin O-acyltransferase, 231-, Membrane-bound O-acyltransferase domain-containing protein 4, O-acyltransferase domain-containing protein 4, MBOAT4, GOAT, OACT4 |
Target/Specificity | This MBOAT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-287 amino acids from the Central region of human MBOAT4. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MBOAT4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MBOAT4 (HGNC:32311) |
---|---|
Synonyms | GOAT, OACT4 |
Function | Catalyzes ghrelin acylation at 'Ser-3' using preferentially octanoyl-CoA, hexanoyl-CoA and decanoyl-CoA as acyl-CoA donors leading to ghrelin activity (PubMed:24045953, PubMed:18443287, PubMed:25562443, PubMed:28134508). In vitro uses also acyl-CoA donors of different lengths from short-chain (C2) to long-chain fatty acids (C16) knowing that acyl-CoA donors from butanoyl-CoA (C4) to dodecanoyl-CoA (C12) are more efficient compared to longer acyl-CoA donors, such as myristoyl- CoA (C14) and palmitoyl-CoA (C16) that are not efficient (PubMed:18443287). |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P0C7A3}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P0C7A3} |
Tissue Location | Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
MBOAT4 mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.
References
Takahashi, T., et al. J. Biochem. 146(5):675-682(2009) Gomez, R., et al. Arthritis Rheum. 60(6):1704-1709(2009) Gutierrez, J.A., et al. Proc. Natl. Acad. Sci. U.S.A. 105(17):6320-6325(2008)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.