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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PCCA Antibody (Center)   

PCCA Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - PCCA Antibody (Center) AP12328c
    AP12328c staining PCCA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PCCA Antibody (Center) AP12328c
    AP12328c staining PCCA in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PCCA Antibody (Center) AP12328c
    AP12328c staining PCCA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PCCA Antibody (Center) AP12328c
    AP12328c staining PCCA in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - PCCA Antibody (Center) AP12328c
    Anti-PCCA Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 80 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PCCA Antibody (Center) AP12328c
    All lanes : Anti-PCCA Antibody (Center) at 1:2000 dilution Lane 1: human liver lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 80 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PCCA Antibody (Center) AP12328c
    All lanes : Anti-PCCA Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: rat cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 80 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PCCA Antibody (Center) AP12328c
    All lanes : Anti-PCCA Antibody (Center) at 1:2000 dilution Lane 1: rat cerebellum lysate Lane 2: human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 80 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PCCA Antibody (Center) AP12328c
    Anti-PCCA Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 80 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PCCA Antibody (Center) AP12328c
    All lanes : Anti-PCCA Antibody (Center) at 1:2000 dilution Lane 1: human liver lysate Lane 2: rat cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 80 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PCCA Antibody (Center) AP12328c
    Anti-PCCA Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 80 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PCCA Antibody (Center) AP12328c
    PCCA Antibody (Center) (Cat. #AP12328c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PCCA antibody detected the PCCA protein (arrow).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P05165
Other Accession NP_001121164.1, NP_000273.2
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 80059 Da
Antigen Region 362-390 aa
Additional Information
Gene ID 5095
Other Names Propionyl-CoA carboxylase alpha chain, mitochondrial, PCCase subunit alpha, Propanoyl-CoA:carbon dioxide ligase subunit alpha, PCCA
Target/Specificity This PCCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-390 amino acids from the Central region of human PCCA.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCCA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCCA
Cellular Location Mitochondrion matrix
Research Areas
Citations (0)

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Background

The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Huang, C.S., et al. Nature 466(7309):1001-1005(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
MacDonald, M.J., et al. Diabetologia 52(6):1087-1091(2009)

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$ 295.00
$ 99.00
Cat# AP12328c
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Quantity:
(40 western blots)
Availability: In Stock
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