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FOXO6 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FOXO6 Antibody (Center) AP12340C
    FOXO6 Antibody (Center) (Cat. #AP12340c) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the FOXO6 antibody detected the FOXO6 protein (arrow).
  • IF - FOXO6 Antibody (Center) AP12340C
    Fluorescent confocal image of U251 cell stained with FOXO6 Antibody (Center)(Cat#AP12340c).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXO6 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXO6 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession A8MYZ6
Other Accession Q70KY4, XP_002342143.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50595 Da
Antigen Region 154-181 aa
Additional Information
Other Names Forkhead box protein O6, FOXO6
Target/Specificity This FOXO6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-181 amino acids from the Central region of human FOXO6.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOXO6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXO6
Function Transcriptional activator.
Cellular Location Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089}. Note=When phosphorylated, translocated from nucleus to cytoplasm. High nuclear localization after stimulation with growth factors (By similarity)
Citations (0)

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Background

FOXO6 is a transcriptional activator (By similarity).

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Cat# AP12340C
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(40 western blots)
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