|Application ||WB, E|
|Other Accession||NP_001013859.1, NP_001013858.1, NP_003541.2|
|Calculated MW||83067 Da|
|Antigen Region||124-151 aa|
|Other Names||Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, MAD1-like protein 1, Mitotic checkpoint MAD1 protein homolog, HsMAD1, hMAD1, Tax-binding protein 181, MAD1L1, MAD1, TXBP181|
|Target/Specificity||This MAD1L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-151 amino acids from the N-terminal region of human MAD1L1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MAD1L1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore|
|Tissue Location||Expressed weakly at G0/G1 and highly at late S and G2/M phase|
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Provided below are standard protocols that you may find useful for product applications.
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Guo, Y., et al. J. Med. Genet. 47(9):616-622(2010)
Wang, H.B., et al. J. Gastrointest. Surg. 14(8):1227-1234(2010)
Hewitt, L., et al. J. Cell Biol. 190(1):25-34(2010)
Ge, Z., et al. FASEB J. 24(2):579-586(2010)
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