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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   PIGA Antibody (C-term)   

PIGA Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PIGA Antibody (C-term) AP12378b
    PIGA Antibody (C-term) (Cat. #AP12378b) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PIGA antibody detected the PIGA protein (arrow).
  • IHC-P - PIGA Antibody (C-term) AP12378b
    PIGA Antibody (C-term) (Cat. #AP12378b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P37287
Other Accession NP_065206.3, NP_002632.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54127 Da
Antigen Region 455-484 aa
Additional Information
Gene ID 5277
Other Names Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, GlcNAc-PI synthesis protein, Phosphatidylinositol-glycan biosynthesis class A protein, PIG-A, PIGA
Target/Specificity This PIGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-484 amino acids from the C-terminal region of human PIGA.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIGA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIGA
Function Necessary for the synthesis of N-acetylglucosaminyl- phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein
Research Areas
Citations (0)

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Background

This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12.

References

Borowitz, M.J., et al. Cytometry B Clin Cytom 78(4):211-230(2010)
Peruzzi, B., et al. Mutat. Res. 705(1):3-10(2010)
Araten, D.J., et al. Mutat. Res. 686 (1-2), 1-8 (2010) :
Iida, Y., et al. Blood 83(11):3126-3131(1994)
Ware, R.E., et al. Blood 83(9):2418-2422(1994)

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$ 295.00
$ 99.00
Cat# AP12378b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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