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CKAP4 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q07065 |
| Other Accession | NP_006816.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 66022 Da |
| Antigen Region | 574-602 aa |
| Gene ID | 10970 |
|---|---|
| Other Names | Cytoskeleton-associated protein 4, 63-kDa cytoskeleton-linking membrane protein, Climp-63, p63, CKAP4 |
| Target/Specificity | This CKAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 574-602 amino acids from the C-terminal region of human CKAP4. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CKAP4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CKAP4 |
|---|---|
| Function | Mediates the anchoring of the endoplasmic reticulum to microtubules. |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region Note=Translocates to the perinuclear region upon APF-stimulation |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
CKAP4 is a substrate of DHHC2, a putative tumor suppressor. CK MNF116 and CKAP4 were useful in identifying squamous cell carcinomas with single cell infiltration. These results suggest a role for CKAP4 in tumor.
References
Kanner, W.A., et al. J. Cutan. Pathol. 37(7):744-750(2010)
Tomkova, H., et al. Eur J Dermatol 20(3):411-413(2010)
Lefkimmiatis, K., et al. Cancer Res. 69(22):8563-8571(2009)
Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009)
Bates, S.R., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (4), L658-L669 (2008) :
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If you have any additional inquiries please email technical services at tech@abcepta.com.
Anonymous
2016-08-23 21:15:32
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Species tested
Human
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose |
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