|Application ||IHC-P, WB, E|
|Calculated MW||66022 Da|
|Antigen Region||574-602 aa|
|Other Names||Cytoskeleton-associated protein 4, 63-kDa cytoskeleton-linking membrane protein, Climp-63, p63, CKAP4|
|Target/Specificity||This CKAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 574-602 amino acids from the C-terminal region of human CKAP4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CKAP4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||High-affinity epithelial cell surface receptor for APF.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Note=Translocates to the perinuclear region upon APF- stimulation|
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Provided below are standard protocols that you may find useful for product applications.
CKAP4 is a substrate of DHHC2, a putative tumor suppressor. CK MNF116 and CKAP4 were useful in identifying squamous cell carcinomas with single cell infiltration. These results suggest a role for CKAP4 in tumor.
Kanner, W.A., et al. J. Cutan. Pathol. 37(7):744-750(2010)
Tomkova, H., et al. Eur J Dermatol 20(3):411-413(2010)
Lefkimmiatis, K., et al. Cancer Res. 69(22):8563-8571(2009)
Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009)
Bates, S.R., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (4), L658-L669 (2008) :
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1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose