ST6GALNAC1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9NSC7 |
Other Accession | NP_060884.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 68564 Da |
Antigen Region | 28-57 aa |
Gene ID | 55808 |
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Other Names | Alpha-N-acetylgalactosaminide alpha-2, 6-sialyltransferase 1, GalNAc alpha-2, 6-sialyltransferase I, ST6GalNAc I, ST6GalNAcI, Sialyltransferase 7A, SIAT7-A, ST6GALNAC1, SIAT7A |
Target/Specificity | This ST6GALNAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the N-terminal region of human ST6GALNAC1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ST6GALNAC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ST6GALNAC1 (HGNC:23614) |
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Synonyms | SIAT7A |
Function | Protein sialyltransferase specifically expressed in goblet cells that plays a key role in intestinal host-commensal homeostasis (PubMed:35303419). Conjugates sialic acid with an alpha-2-6 linkage to N-acetylgalactosamine (GalNAc) glycan chains linked to serine or threonine in glycoproteins (PubMed:16319059, PubMed:35303419). Catalyzes the formation of the sialyl-Tn (S-Tn) antigen, an antigen found in intestinal goblet cells, as well as ulcerative colitis (UC) and various cancers (PubMed:16319059, PubMed:35303419). Protein sialylation in globlet cells is essential for mucus integrity and is required to protect the intestinal mucus against excessive bacterial proteolytic degradation (PubMed:35303419). |
Cellular Location | Golgi apparatus membrane; Single-pass type II membrane protein |
Tissue Location | Expression is restricted to the gastrointestinal tract (PubMed:16319059). Highly expressed in goblet cells (PubMed:35303419). Also expressed in various tumor cells (PubMed:16319059). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Glycosylation of proteins affects cell-cell interaction, interactions with the matrix, and the functions of intracellular molecules. ST6GALNAC1 transfers a sialic acid, N-acetylneuraminic acid (NeuAc), in an alpha-2,6 linkage to O-linked GalNAc residues. The cancer-associated sialyl-Tn (sTn) antigen is formed by ST6GALNAC1-catalyzed sialylation of GalNAc residues on mucins (Ikehara et al., 1999 [PubMed 10536037]; Sewell et al., 2006 [PubMed 16319059]).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Hassinen, A., et al. J. Biol. Chem. 285(23):17771-17777(2010)
Patani, N., et al. Cancer Genomics Proteomics 5(6):333-340(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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