- CITATIONS: 4
|Application ||WB, IHC-P, E|
|Calculated MW||126146 Da|
|Antigen Region||1081-1112 aa|
|Other Names||Sentrin-specific protease 6, SUMO-1-specific protease 1, Sentrin/SUMO-specific protease SENP6, SENP6, KIAA0797, SSP1, SUSP1|
|Target/Specificity||This SENP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1081-1112 amino acids from the C-terminal region of human SENP6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SENP6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA0797, SSP1, SUSP1|
|Function||Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly- SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.|
|Tissue Location||Highly expressed in reproductive organs, such as testis, ovary and prostate|
Provided below are standard protocols that you may find useful for product applications.
SENP6 (SUSP1) is a protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. This protein does not seem to be involved in the processing of full-length SUMO proteins to their mature conjugatable forms. SENP6 deconjugates SUMO1 from RXRA, leading to transcriptional activation. It may act preferentially on substrates containing 3 or more SUMO2 or SUMO3 moieties.
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Kim, K.I., et al., J. Biol. Chem. 275(19):14102-14106 (2000).
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