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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   VSGP/F-spondin Antibody (N-term)   

VSGP/F-spondin Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - VSGP/F-spondin Antibody (N-term) AP12414a
    All lanes : Anti-VSGP/F-spondin Antibody (N-term) at 1:2000 dilution Lane 1: Human heart lysate Lane 2: Human lung lysate Lane 3: Mouse lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - VSGP/F-spondin Antibody (N-term) AP12414a
    VSGP/F-spondin Antibody (N-term) (Cat. #AP12414a) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the VSGP/F-spondin antibody detected the VSGP/F-spondin protein (arrow).
  • IHC-P - VSGP/F-spondin Antibody (N-term) AP12414a
    VSGP/F-spondin Antibody (N-term) (Cat. #AP12414a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VSGP/F-spondin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9HCB6
Other Accession Q9GLX9, NP_006099.2
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 90973 Da
Antigen Region 15-43 aa
Additional Information
Gene ID 10418
Other Names Spondin-1, F-spondin, Vascular smooth muscle cell growth-promoting factor, SPON1, KIAA0762, VSGP
Target/Specificity This VSGP/F-spondin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human VSGP/F-spondin.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVSGP/F-spondin Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPON1
Synonyms KIAA0762, VSGP
Function Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen
Research Areas
Citations (0)

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Background

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.

References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Attur, M.G., et al. FASEB J. 23(1):79-89(2009)
Tan, K., et al. J. Mol. Biol. 381(5):1213-1223(2008)
Kitagawa, M., et al. Biochem. Biophys. Res. Commun. 349(3):1050-1056(2006)
Pyle-Chenault, R.A., et al. Tumour Biol. 26(5):245-257(2005)

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$ 295.00
$ 99.00
Cat# AP12414a
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(40 western blots)
Availability: In Stock
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