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TCF7L2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TCF7L2 Antibody (N-term) AP12416A
    Anti-TCF7L2 Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TCF7L2 Antibody (N-term) AP12416A
    All lanes : Anti-TCF7L2 Antibody (N-term) at 1:2000 dilution Lane 1: HCT116 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: mouse cerebellum lysate Lane 5: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TCF7L2 Antibody (N-term) AP12416A
    TCF7L2 Antibody (N-term) (Cat. #AP12416a) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TCF7L2 antibody detected the TCF7L2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NQB0
Other Accession Q924A0, NP_001139756.1, NP_001139746.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 67919 Da
Antigen Region 61-90 aa
Additional Information
Gene ID 6934
Other Names Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4, TCF7L2, TCF4
Target/Specificity This TCF7L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the N-terminal region of human TCF7L2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTCF7L2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCF7L2
Synonyms TCF4
Function Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
Cellular Location Nucleus, PML body. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies
Tissue Location Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.
Research Areas
Citations (0)

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Background

This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.

References

Hansson, O., et al. Curr. Diab. Rep. 10(6):444-451(2010)
Heni, M., et al. Diabetes (2010) In press :
Potapov, V.A., et al. Genetika 46(8):1123-1131(2010)
Kucharska-Newton, A.M., et al. J Obes 2010 (2010) :
Zabaneh, D., et al. PLoS ONE 5 (8), E11961 (2010) :

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$ 295.00
$ 99.00
Cat# AP12416A
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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