|Application ||WB, E|
|Other Accession||Q924A0, NP_001139756.1, NP_001139746.1|
|Calculated MW||67919 Da|
|Antigen Region||61-90 aa|
|Other Names||Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4, TCF7L2, TCF4|
|Target/Specificity||This TCF7L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the N-terminal region of human TCF7L2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TCF7L2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.|
|Cellular Location||Nucleus, PML body. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies|
|Tissue Location||Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.
Hansson, O., et al. Curr. Diab. Rep. 10(6):444-451(2010)
Heni, M., et al. Diabetes (2010) In press :
Potapov, V.A., et al. Genetika 46(8):1123-1131(2010)
Kucharska-Newton, A.M., et al. J Obes 2010 (2010) :
Zabaneh, D., et al. PLoS ONE 5 (8), E11961 (2010) :
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