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MYBPC3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - MYBPC3 Antibody (N-term) AP12436a
    AP12436A staining MYBPC3 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - MYBPC3 Antibody (N-term) AP12436a
    AP12436A staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - MYBPC3 Antibody (N-term) AP12436a
    AP12436A staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - MYBPC3 Antibody (N-term) AP12436a
    All lanes : Anti-MYBPC3 Antibody (N-term) at 1:2000 dilution Lane 1: mouse heart lysate Lane 2: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 141 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MYBPC3 Antibody (N-term) AP12436a
    All lanes : Anti-MYBPC3 Antibody (N-term) at 1:1000-1:2000 dilution Lane 1: human liver lysate Lane 2: mouse heart lysate Lane 3: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 141 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MYBPC3 Antibody (N-term) AP12436a
    Western blot analysis of lysate from rat heart tissue lysate, using MYBPC3 Antibody (N-term)(Cat. #AP12436a). AP12436a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - MYBPC3 Antibody (N-term) AP12436a
    Western blot analysis of lysate from mouse heart tissue lysate, using MYBPC3 Antibody (N-term)(Cat. #AP12436a). AP12436a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q14896
Other Accession P56741, O70468, NP_000247.2
Reactivity Human, Mouse, Rat
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 140762 Da
Antigen Region 189-218 aa
Additional Information
Gene ID 4607
Other Names Myosin-binding protein C, cardiac-type, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC3
Target/Specificity This MYBPC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the N-terminal region of human MYBPC3.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMYBPC3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYBPC3
Function Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
Research Areas
Citations (0)

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Background

MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy.

References

Millat, G., et al. Clin. Chim. Acta 411 (23-24), 1983-1991 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Millat, G., et al. Eur J Med Genet 53(5):261-267(2010)
Zimmerman, R.S., et al. Genet. Med. 12(5):268-278(2010)
Brion, M., et al. Ann. Clin. Lab. Sci. 40(3):285-289(2010)

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$ 295.00
$ 99.00
Cat# AP12436a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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