|Application ||WB, E|
|Calculated MW||80311 Da|
|Antigen Region||237-265 aa|
|Other Names||SUN domain-containing protein 2, Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2, SUN2, FRIGG, KIAA0668, RAB5IP, UNC84B|
|Target/Specificity||This UNC84B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-265 amino acids from the Central region of human UNC84B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UNC84B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||FRIGG, KIAA0668, RAB5IP, UNC84B|
|Function||Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5.|
|Cellular Location||Nucleus inner membrane; Single-pass type II membrane protein. Endosome membrane; Single-pass type II membrane protein|
|Tissue Location||Widely expressed. Highly expressed in heart, lung and muscle. Weakly expressed in fetal heart. Slightly overexpressed in some heart tissues form patients with congenital heart defects.|
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Provided below are standard protocols that you may find useful for product applications.
SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).[supplied by OMIM].
Turgay, Y., et al. EMBO J. 29(14):2262-2275(2010)
Haque, F., et al. J. Biol. Chem. 285(5):3487-3498(2010)
Stewart-Hutchinson, P.J., et al. Exp. Cell Res. 314(8):1892-1905(2008)
Cerhan, J.R., et al. Blood 110(13):4455-4463(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
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