- CITATIONS: 1
|Application ||WB, IHC-P, E|
|Other Accession||NP_001155253.1, NP_001155252.1|
|Calculated MW||61054 Da|
|Antigen Region||308-336 aa|
|Other Names||[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, PDP 1, Protein phosphatase 2C, Pyruvate dehydrogenase phosphatase catalytic subunit 1, PDPC 1, PDP1, PDP, PPM2C|
|Target/Specificity||This PDP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the Central region of human PDP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PDP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.|
|Cellular Location||Mitochondrion matrix.|
Provided below are standard protocols that you may find useful for product applications.
Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Kato, J., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 3), 342-345 (2010) :
Cameron, J.M., et al. Hum. Genet. 125(3):319-326(2009)
Stellingwerff, T., et al. Am. J. Physiol. Endocrinol. Metab. 290 (2), E380-E388 (2006) :
Maj, M.C., et al. J. Clin. Endocrinol. Metab. 90(7):4101-4107(2005)
Piccinini, M., et al. Obes. Res. 13(4):678-686(2005)
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