|Application ||WB, IHC-P, E|
|Other Accession||O88904, NP_938009.1, NP_938010.1|
|Calculated MW||130843 Da|
|Antigen Region||893-922 aa|
|Other Names||Homeodomain-interacting protein kinase 1, Nuclear body-associated kinase 2, HIPK1, KIAA0630, MYAK, NBAK2|
|Target/Specificity||This HIPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 893-922 amino acids from the C-terminal region of human HIPK1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HIPK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA0630, MYAK, NBAK2|
|Function||Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti- oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation.|
|Cellular Location||Nucleus. Cytoplasm. Note=Predominantly nuclear. Translocates from nucleus to cytoplasm in response to stress stimuli via SENP1-mediated desumoylation|
|Tissue Location||Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Isoform 2 is highly expressed in testis|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms.
Matre, V., et al. Biochem. Biophys. Res. Commun. 388(1):150-154(2009)
Li, X., et al. J. Biol. Chem. 280(15):15061-15070(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Song, J.J., et al. J. Biol. Chem. 278(47):47245-47252(2003)
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