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Sestrin-3 Antibody (Center Y335)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Sestrin-3 Antibody (Center Y335) AP12471c
    Anti-Sestrin-3 Antibody (Center Y335) at 1:2000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Sestrin-3 Antibody (Center Y335) AP12471c
    Sestrin-3 Antibody (Center Y335) (Cat. #AP12471c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Sestrin-3 antibody detected the Sestrin-3 protein (arrow).
  • IHC-P - Sestrin-3 Antibody (Center Y335) AP12471c
    Sestrin-3 Antibody (Center Y335) (Cat. #AP12471c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Sestrin-3 Antibody (Center Y335) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P58005
Other Accession Q9CYP7, NP_653266.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57291 Da
Antigen Region 320-347 aa
Additional Information
Gene ID 143686
Other Names Sestrin-3, SESN3, SEST3
Target/Specificity This Sestrin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-347 amino acids from the Central region of human Sestrin-3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSestrin-3 Antibody (Center Y335) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SESN3 (HGNC:23060)
Function May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway (PubMed:25263562). May also regulate the insulin-receptor signaling pathway through activation of TORC2 (By similarity). This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity).
Cellular Location Cytoplasm.
Tissue Location Widely expressed.
Research Areas
Citations ( 0 )


Sestrins form a small family of redox enzymes which regulate accumulation of Reactive Oxygen Species. They protect cells and their DNA against oxidative damage and regulate cell growth and viability. These genes are often deregulated in cancers and their inactivation accelerates the growth of model tumors in mice.


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Chen, C.C., et al. Dev. Cell 18(4):592-604(2010)
Peeters, H., et al. Hum. Genet. 112 (5-6), 573-580 (2003) :

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$ 295.00
$ 99.00
Cat# AP12471c
(40 western blots)
Availability: In Stock
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