|Application ||WB, E|
|Calculated MW||50575 Da|
|Antigen Region||23-51 aa|
|Other Names||Diphthamide biosynthesis protein 7, WD repeat-containing protein 85, DPH7, C9orf112, WDR85|
|Target/Specificity||This WDR85 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human WDR85.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||WDR85 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the demethylation of diphthine methyl ester to form diphthine, an intermediate diphthamide biosynthesis, a post- translational modification of histidine which occurs in translation elongation factor 2 (EEF2) which can be ADP- ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
WDR85 is a WD repeat-containing protein that plays a role in the first step of diphthamide biosynthesis (Carette et al., 2009 [PubMed 19965467]).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Carette, J.E., et al. Science 326(5957):1231-1235(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Humphray, S.J., et al. Nature 429(6990):369-374(2004)
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