|Application ||WB, E|
|Calculated MW||50575 Da|
|Antigen Region||23-51 aa|
|Other Names||Diphthamide biosynthesis protein 7, WD repeat-containing protein 85, DPH7, C9orf112, WDR85|
|Target/Specificity||This WDR85 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human WDR85.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||WDR85 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the demethylation of diphthine methyl ester to form diphthine, an intermediate diphthamide biosynthesis, a post- translational modification of histidine which occurs in translation elongation factor 2 (EEF2) which can be ADP- ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta).|
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Provided below are standard protocols that you may find useful for product applications.
WDR85 is a WD repeat-containing protein that plays a role in the first step of diphthamide biosynthesis (Carette et al., 2009 [PubMed 19965467]).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Carette, J.E., et al. Science 326(5957):1231-1235(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Humphray, S.J., et al. Nature 429(6990):369-374(2004)
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