|Application ||WB, IHC-P, E|
|Calculated MW||97802 Da|
|Antigen Region||537-565 aa|
|Other Names||Gamma-secretase-activating protein, GSAP, Protein pigeon homolog, Gamma-secretase-activating protein 16 kDa C-terminal form, GSAP-16K, GSAP, PION|
|Target/Specificity||This PION antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-565 amino acids from the C-terminal region of human PION.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PION Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulator of gamma-secretase activity, which specifically activates the production of beta-amyloid protein (beta-amyloid protein 40 and beta-amyloid protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate beta- amyloid, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD.|
|Cellular Location||Golgi apparatus, trans-Golgi network|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Accumulation of neurotoxic amyloid-beta is a major hallmark of Alzheimer disease (AD; MIM 104300). Formation of amyloid-beta is catalyzed by gamma-secretase (see PSEN1; MIM 104311), a protease with numerous substrates. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate, the amyloid-beta precursor protein (APP; MIM 104760) C-terminal fragment (APP-CTF) (He et al., 2010 [PubMed 20811458]).
He, G., et al. Nature 467(7311):95-98(2010)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
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