- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
PIAS2 (PIASx1) Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP1248a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
PIAS2 (PIASx1) Antibody (C-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | O75928 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 68240 Da |
PIAS2 (PIASx1) Antibody (C-term) - Additional info | |
| Gene ID 9063 | |
| Other Names PIAS2; PIASX; E3 SUMO-protein ligase PIAS2; Androgen receptor-interacting protein 3; DAB2-interacting protein; Msx-interacting zinc finger protein; PIAS-NY protein; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2 | |
| Target/Specificity This PIAS2 (PIASx1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540~570 amino acids from the C-terminal region of human PIASx1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PIAS2 (PIASx1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PIAS2 (PIASx1) Antibody (C-term) - Protein Information | |
| Name PIAS2 | |
| Synonyms PIASX | |
| Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2- alpha | |
| Cellular Location Nucleus speckle. Nucleus, PML body. Note=Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) | |
| Tissue Location Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs | |
PIAS2 (PIASx1) Antibody (C-term) - Related products
AP1280b: PIAS2 (PIASny) Antibody (Center)
AP1246a: PIAS2 (PIASx1/2) Antibody (N-term)
AP1247a: PIAS2 (PIASx1/2) Antibody (C-term)
AP1248a: PIAS2 (PIASx1) Antibody (C-term)
RI13978: PIAS2 predesign siRNA
DC04039: Human PIAS2 cDNA Clone
LY11595a: PIAS2 Over-expression Lysate
BP1246a: PIASx1/2 Antibody (N-term) Blocking Peptide
BP1247a: PIASx1/2 Antibody (C-term) Blocking Peptide
PIAS2 (PIASx1) Antibody (C-term) - Application data
-
Western blot analysis of PIASx1 polyclonal antibody (Cat. #AP1248a) with bacterial and HL-60 cell lysates. Lane A: endogenous PIASx1 in HL-60 cells; Lane B: bacteria expressing recombinant GST-PIASx1 fusion protein. PIASx1 was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
-
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PIAS2 (PIASx1) Antibody (C-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PIASX functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. PIASX binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. This antibody is directed against an epitope specific to PIAS2-alpha. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.
REFERENCES
Arora, T., et al., J. Biol. Chem. 278(24):21327-21330 (2003). Wu, S., et al., Oncogene 22(3):351-360 (2003). Liu, B., et al., Proc. Natl. Acad. Sci. U.S.A. 95(18):10626-10631 (1998). Wu, L., et al., Mech. Dev. 65 (1-2), 3-17 (1997).
