|Application ||WB, IHC-P, FC, E|
|Other Accession||NP_490647.1, NP_005485.1|
|Calculated MW||36087 Da|
|Antigen Region||218-247 aa|
|Other Names||DnaJ homolog subfamily B member 6, HHDJ1, Heat shock protein J2, HSJ-2, MRJ, MSJ-1, DNAJB6, HSJ2, MRJ, MSJ1|
|Target/Specificity||This DNAJB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-247 amino acids from the Central region of human DNAJB6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DNAJB6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HSJ2, MRJ, MSJ1|
|Function||Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.|
|Cellular Location||Cytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line|
|Tissue Location||Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.
Mitra, A., et al. J. Biol. Chem. 285(32):24686-24694(2010)
Edo De Bock, C., et al. Int. J. Oncol. 36(5):1155-1163(2010)
Hageman, J., et al. Mol. Cell 37(3):355-369(2010)
Lowe, J.K., et al. PLoS Genet. 5 (2), E1000365 (2009) :
Dey, S., et al. Mol. Cell. Biochem. 322 (1-2), 137-142 (2009) :
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