|Application ||WB, IHC-P, E|
|Other Accession||P52873, Q05920, Q29RK2, NP_001035806.1, NP_000911.2|
|Calculated MW||129634 Da|
|Antigen Region||53-82 aa|
|Other Names||Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase, PCB, PC|
|Target/Specificity||This PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-82 amino acids from the N-terminal region of human PC.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.|
|Cellular Location||Mitochondrion matrix.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq].
MacDonald, M.J., et al. Diabetologia 52(6):1087-1091(2009)
Monnot, S., et al. Hum. Mutat. 30(5):734-740(2009)
Wang, D., et al. Mol. Genet. Metab. 95 (1-2), 31-38 (2008) :
Vora, S., et al. Natl Med J India 21(3):116-119(2008)
Xiang, S., et al. Nat. Struct. Mol. Biol. 15(3):295-302(2008)
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