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PC Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PC Antibody (N-term) AP12494A
    Anti-PC Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 130 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PC Antibody (N-term) AP12494A
    PC Antibody (N-term) (Cat. #AP12494a) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PC antibody detected the PC protein (arrow).
  • IHC-P - PC Antibody (N-term) AP12494A
    PC Antibody (N-term) (Cat. #AP12494a)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11498
Other Accession P52873, Q05920, Q29RK2, NP_001035806.1, NP_000911.2
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 129634 Da
Antigen Region 53-82 aa
Additional Information
Gene ID 5091
Other Names Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase, PCB, PC
Target/Specificity This PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-82 amino acids from the N-terminal region of human PC.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PC
Function Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)

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This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq].


MacDonald, M.J., et al. Diabetologia 52(6):1087-1091(2009)
Monnot, S., et al. Hum. Mutat. 30(5):734-740(2009)
Wang, D., et al. Mol. Genet. Metab. 95 (1-2), 31-38 (2008) :
Vora, S., et al. Natl Med J India 21(3):116-119(2008)
Xiang, S., et al. Nat. Struct. Mol. Biol. 15(3):295-302(2008)

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$ 295.00
$ 99.00
Cat# AP12494A
(40 western blots)
Availability: In Stock
Bulk Size
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