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POLR1B Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - POLR1B Antibody (N-term) AP12495a
    POLR1B Antibody (N-term) (Cat. #AP12495a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the POLR1B antibody detected the POLR1B protein (arrow).
  • IHC-P - POLR1B Antibody (N-term) AP12495a
    POLR1B Antibody (N-term) (Cat. #AP12495a)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLR1B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - POLR1B Antibody (N-term) AP12495a
    POLR1B Antibody (N-term) (Cat. #AP12495a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H9Y6
Other Accession O54888, NP_061887.2, NP_001131076.1
Reactivity Human
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 128229 Da
Antigen Region 89-117 aa
Additional Information
Gene ID 84172
Other Names DNA-directed RNA polymerase I subunit RPA2, RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135, POLR1B
Target/Specificity This POLR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the N-terminal region of human POLR1B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLR1B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).
Cellular Location Nucleus, nucleolus
Citations (0)

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Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).


Wen, L., et al. Biochem. Biophys. Res. Commun. 367(4):846-851(2008)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
Panova, T.B., et al. Mol. Cell. Biol. 26(16):5957-5968(2006)
Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006)

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Cat# AP12495a
(40 western blots)
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