|Application ||WB, IHC-P, FC, E|
|Other Accession||O54888, NP_061887.2, NP_001131076.1|
|Calculated MW||128229 Da|
|Antigen Region||89-117 aa|
|Other Names||DNA-directed RNA polymerase I subunit RPA2, RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135, POLR1B|
|Target/Specificity||This POLR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the N-terminal region of human POLR1B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||POLR1B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).|
|Cellular Location||Nucleus, nucleolus|
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Provided below are standard protocols that you may find useful for product applications.
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).
Wen, L., et al. Biochem. Biophys. Res. Commun. 367(4):846-851(2008)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
Panova, T.B., et al. Mol. Cell. Biol. 26(16):5957-5968(2006)
Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006)
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