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PIASy1 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP1250a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
PIASy1 Antibody (C-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q8N2W9 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 56504 Da |
PIASy1 Antibody (C-term) - Additional info | |
| Gene ID 51588 | |
| Other Names PIAS4; PIASG; E3 SUMO-protein ligase PIAS4; PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma | |
| Target/Specificity This PIASy1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 478~507 amino acids from the C-terminal region of human PIASy1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PIASy1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PIASy1 Antibody (C-term) - Protein Information | |
| Name PIAS4 | |
| Synonyms PIASG | |
| Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations | |
| Cellular Location Nucleus, PML body. Note=Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies | |
| Tissue Location Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes | |
PIASy1 Antibody (C-term) - Related products
AP1249a: PIASy Antibody (N-term)
AP1250a: PIASy1 Antibody (C-term)
RI13980: PIAS4 predesign siRNA
PIASy1 Antibody (C-term) - Application data
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The PIASy1 polyclonal antibody (Cat. #AP1250a) is used in Western blot to detect recombinant GST-PIASy1 in bacterial cell lysate.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PIASy1 Antibody (C-term) - Research Areas
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BACKGROUND
PIASy1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway, the wnt pathway and the steroid hormone signaling pathway. PIASy1 is involved in gene silencing, and promotes PARK7 sumoylation.
REFERENCES
Imoto, S., et al., J. Biol. Chem. 278(36):34253-34258 (2003). Chun, T.H., et al., Circ. Res. 92(11):1201-1208 (2003). Subramanian, L., et al., J. Biol. Chem. 278(11):9134-9141 (2003). Liu, B., et al., Proc. Natl. Acad. Sci. U.S.A. 98(6):3203-3207 (2001). Liu, B., et al., Proc. Natl. Acad. Sci. U.S.A. 95(18):10626-10631 (1998).
