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Mdm2 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Alternative | |||
| AP1253a | AP1254a, AP1253d, AP1253e | DISCONTINED INQUIRE CLICK INQUIRE Add to cart | ||
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Mdm2 Antibody (N-term) - Product info | |
| Application | WB, IHC, FC
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| Primary Accession | Q00987 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 55233 Da |
Mdm2 Antibody (N-term) - Additional info | |
| Gene ID 4193 | |
| Other Names MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Oncoprotein Mdm2; p53-binding protein Mdm2 | |
| Target/Specificity This Mdm2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-250 amino acids from the N-terminal region of human Mdm2. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Mdm2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Mdm2 Antibody (N-term) - Protein Information | |
| Name MDM2 | |
| Function E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation | |
| Cellular Location Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus | |
| Tissue Location Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues | |
Mdm2 Antibody (N-term) - Related products
AP1253a: Mdm2 Antibody (N-term)
AP1254a: Mdm2 Antibody (C-term)
AP3490a: Phospho-MDM2-T218 Antibody
AP3501a: Phospho-MDM2-pS166 Antibody
AP3579a: Phospho-MDM2-pS395 Antibody
BP1253a: Mdm2 Antibody (N-term) Blocking Peptide
BP1253d: MDM2 Antibody (T218) Blocking Peptide
BP1253e: MDM2 Antibody (S166) Blocking Peptide
BP1254a: Mdm2 Antibody (C-term) Blocking Peptide
BP3490a: Phospho-MDM2-T218 Antibody Blocking Peptide
BP3501a: Phospho-MDM2-pS166 Antibody Blocking Peptide
Mdm2 Antibody (N-term) - Application data
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Western blot analysis of anti-Mdm2 Pab (Cat. #AP1253a) in mouse kidney tissue lysate. Mdm2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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Formalin-fixed and paraffin-embedded human colon carcinoma with Mdm2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of WiDr cells using Mdm2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Mdm2 Antibody (N-term) - Research Areas
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BACKGROUND
MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
REFERENCES
Burch, L.R., et al., J. Mol. Biol. 337(1):115-128 (2004). Schon, O., et al., J. Mol. Biol. 336(1):197-202 (2004). Mantesso, A., et al., J. Oral Pathol. Med. 33(2):96-101 (2004). Shmueli, A., et al., Mol. Cell 13(1):4-5 (2004). Xia, L., et al., Cancer Res. 64(1):221-228 (2004).
