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UBTF Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P17480 |
| Other Accession | NP_001070152.1, NP_055048.1, NP_001070151.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 89406 Da |
| Antigen Region | 631-660 aa |
| Gene ID | 7343 |
|---|---|
| Other Names | Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1, UBTF, UBF, UBF1 |
| Target/Specificity | This UBTF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 631-660 amino acids from the C-terminal region of human UBTF. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | UBTF Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | UBTF |
|---|---|
| Synonyms | UBF, UBF1 |
| Function | Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I (Pol I) through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element and can activate Pol I promoter escape. |
| Cellular Location | Nucleus, nucleolus {ECO:0000250|UniProtKB:P25976} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).
References
Hisaoka, M., et al. Mol. Cell. Biol. 30(20):4952-4964(2010)
Liu, Z., et al. Proteins 77(2):432-447(2009)
Lin, C.I., et al. Biochem. Biophys. Res. Commun. 386(2):396-401(2009)
Grob, A., et al. J. Cell. Sci. 122 (PT 4), 489-498 (2009) :
He, J., et al. Folia Biol. (Praha) 54(6):202-206(2008)
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