|Application ||WB, E|
|Other Accession||NP_001070152.1, NP_055048.1, NP_001070151.1|
|Calculated MW||89406 Da|
|Antigen Region||631-660 aa|
|Other Names||Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1, UBTF, UBF, UBF1|
|Target/Specificity||This UBTF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 631-660 amino acids from the C-terminal region of human UBTF.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBTF Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.|
|Cellular Location||Nucleus, nucleolus.|
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Provided below are standard protocols that you may find useful for product applications.
Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).
Hisaoka, M., et al. Mol. Cell. Biol. 30(20):4952-4964(2010)
Liu, Z., et al. Proteins 77(2):432-447(2009)
Lin, C.I., et al. Biochem. Biophys. Res. Commun. 386(2):396-401(2009)
Grob, A., et al. J. Cell. Sci. 122 (PT 4), 489-498 (2009) :
He, J., et al. Folia Biol. (Praha) 54(6):202-206(2008)
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