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NDUFS8 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NDUFS8 Antibody (Center) AP12552c
    NDUFS8 Antibody (Center) (Cat. #AP12552c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NDUFS8 antibody detected the NDUFS8 protein (arrow).
  • IHC-P - NDUFS8 Antibody (Center) AP12552c
    NDUFS8 Antibody (Center) (Cat. #AP12552c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDUFS8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00217
Other Accession Q8K3J1, Q60HE3, Q22619, P42028, NP_002487.1
Reactivity Human
Predicted Bovine, C.Elegans, Monkey, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 23705 Da
Antigen Region 81-108 aa
Additional Information
Gene ID 4728
Other Names NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, Complex I-23kD, CI-23kD, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY subunit, NDUFS8
Target/Specificity This NDUFS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the Central region of human NDUFS8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFS8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.
Cellular Location Mitochondrion.
Research Areas
Citations (0)

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This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome.


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : Bourges, I., et al. Biochem. J. 383 (PT 3), 491-499 (2004) : Procaccio, V., et al. Neurology 62(10):1899-1901(2004) Ugalde, C., et al. Hum. Mol. Genet. 13(6):659-667(2004) Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003)

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Cat# AP12552c
(40 western blots)
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