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DAZ4 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DAZ4 Antibody (Center) AP12556c
    DAZ4 Antibody (Center) (Cat. #AP12556c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the DAZ4 antibody detected the DAZ4 protein (arrow).
    detail
  • IHC-P - DAZ4 Antibody (Center) AP12556c
    DAZ4 Antibody (Center) (Cat. #AP12556c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DAZ4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q86SG3
Other Accession Q9NR90, Q13117, Q9NQZ3, NP_001005375.1, NP_065153.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 64785 Da
Antigen Region 90-119 aa
Additional Information
Gene ID 57135
Other Names Deleted in azoospermia protein 4, DAZ4
Target/Specificity This DAZ4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-119 amino acids from the Central region of human DAZ4.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDAZ4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DAZ4
Function RNA-binding protein that plays an essential role in spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Nuclear at some stages of spermatozoide development. Localizes both to the nuclei and cytoplasm of spermatozoide differentiation. Nuclear in fetal gonocytes and in spermatogonial nuclei. It then relocates to the cytoplasm during male meiosis
Tissue Location Testis-specific. Expression restricted to premeiotic germ cells, particularly in spermatogonia (at protein level).
Research Areas
Citations (0)
citation

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Background

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains two copies of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

References

Kim, B., et al. Hum. Reprod. 24(6):1507-1515(2009)
Lardone, M.C., et al. Fertil. Steril. 88(5):1318-1326(2007)
A, Z.C., et al. Yi Chuan 28(9):1057-1060(2006)
Kuo, P.L., et al. Fertil. Steril. 81(4):1034-1040(2004)
Ferlin, A., et al. Fertil. Steril. 81(4):1013-1018(2004)

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$ 365.00
$ 140.00
Cat# AP12556c
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