|Application ||WB, IHC-P, FC, E|
|Other Accession||NP_001165349.1, NP_005360.3|
|Calculated MW||107673 Da|
|Antigen Region||804-833 aa|
|Other Names||Proto-oncogene DBL, Proto-oncogene MCF-2, MCF2-transforming protein, DBL-transforming protein, MCF2, DBL|
|Target/Specificity||This MCF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 804-833 amino acids from the C-terminal region of human MCF2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MCF2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.|
|Cellular Location||Cytoplasm. Isoform 3: Membrane. Note=Colocalizes with CDC42 to plasma membrane|
|Tissue Location||Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.
Piton, A., et al. Mol. Psychiatry (2010) In press :
Murakami, M., et al. Int. J. Cancer 123(3):500-510(2008)
Murakami, M., et al. Cancer Biol. Ther. 7(5):677-688(2008)
Rojas, R.J., et al. J. Biol. Chem. 282(40):29201-29210(2007)
Prag, S., et al. Mol. Biol. Cell 18(8):2935-2948(2007)
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