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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MCF2 Antibody (C-term)   

MCF2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MCF2 Antibody (C-term) AP12563b
    MCF2 Antibody (C-term) (Cat. #AP12563b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MCF2 antibody detected the MCF2 protein (arrow).
  • IHC-P - MCF2 Antibody (C-term) AP12563b
    MCF2 Antibody (C-term) (Cat. #AP12563b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCF2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - MCF2 Antibody (C-term) AP12563b
    MCF2 Antibody (C-term) (Cat. #AP12563b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10911
Other Accession NP_001165349.1, NP_005360.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 107673 Da
Antigen Region 804-833 aa
Additional Information
Gene ID 4168
Other Names Proto-oncogene DBL, Proto-oncogene MCF-2, MCF2-transforming protein, DBL-transforming protein, MCF2, DBL
Target/Specificity This MCF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 804-833 amino acids from the C-terminal region of human MCF2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMCF2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MCF2
Synonyms DBL
Function Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.
Cellular Location Cytoplasm. Isoform 3: Membrane. Note=Colocalizes with CDC42 to plasma membrane
Tissue Location Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis
Research Areas
Citations (0)

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The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.


Piton, A., et al. Mol. Psychiatry (2010) In press :
Murakami, M., et al. Int. J. Cancer 123(3):500-510(2008)
Murakami, M., et al. Cancer Biol. Ther. 7(5):677-688(2008)
Rojas, R.J., et al. J. Biol. Chem. 282(40):29201-29210(2007)
Prag, S., et al. Mol. Biol. Cell 18(8):2935-2948(2007)

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Cat# AP12563b
(40 western blots)
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