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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   PSMB10 Antibody (C-term)   

PSMB10 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PSMB10 Antibody (C-term) AP12569B
    All lanes : Anti-PSMB10 Antibody (C-term) at 1:1000 dilution Lane 1: Daudi whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PSMB10 Antibody (C-term) AP12569B
    PSMB10 Antibody (C-term) (Cat. #AP12569b) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PSMB10 antibody detected the PSMB10 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P40306
Other Accession NP_002792.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28936 Da
Antigen Region 214-242 aa
Additional Information
Gene ID 5699
Other Names Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i, PSMB10, LMP10, MECL1
Target/Specificity This PSMB10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the C-terminal region of human PSMB10.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMB10 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMB10
Synonyms LMP10, MECL1
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
Cellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809}. Nucleus
Research Areas
Citations (0)

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Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

References

Bailey, S.D., et al. Diabetes Care (2010) In press :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Moschonas, A., et al. Mol. Cell. Biol. 28(20):6208-6222(2008)
Liu, Y., et al. DNA Seq. 18(4):257-264(2007)
Listovsky, T., et al. EMBO J. 23(7):1619-1626(2004)

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$ 295.00
$ 99.00
Cat# AP12569B
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Quantity:
(40 western blots)
Availability: In Stock
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