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AOS1 AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1262a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
AOS1 Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q9UBE0 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 38450 Da |
AOS1 Antibody - Additional info | |
| Gene ID 10055 | |
| Other Names SAE1; AOS1; SUA1; UBLE1A; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A | |
| Target/Specificity This AOS1 antibody is generated from rabbits immunized with a recombinant protein encoding full length of human AOS1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AOS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
AOS1 Antibody - Protein Information | |
| Name SAE1 | |
| Synonyms AOS1, SUA1, UBLE1A | |
| Function The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 | |
| Cellular Location Nucleus. | |
| Tissue Location Expression level increases during S phase and drops in G2 phase (at protein level) | |
AOS1 Antibody - Related products
AP1199a: SAE1 (AOS1) Antibody (N-term)
AP1199b: SAE1 (AOS1) Antibody (C-term)
AP2011b: SAE1 (AOS1) Antibody (C-term)
AP2511a: SAE1 (AOS1) Antibody (N-term)
AP2511b: SAE1 (AOS1) Antibody (C-term)
DC00151: Human SAE1 cDNA Clone
LY10831a: SAE1 Over-expression Lysate
BP1199a: SAE1 Antibody (N-term) Blocking Peptide
BP1199b: SAE1 Antibody (C-term) Blocking Peptide
BP2011b: SAE1 Antibody (C-term) Blocking Peptide
BP2511a: AOS1 Antibody (N-term) Blocking Peptide
BP2511b: AOS1 Antibody (C-term) Blocking Peptide
AOS1 Antibody - Application data
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The AOS1 Antibody (Cat. #AP1262a) is used in Western blot to detect AOS1 in mouse colon tissue lysate.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
AOS1 Antibody - Research Areas
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BACKGROUND
The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
REFERENCES
Desterro, J.M., et al., J. Biol. Chem. 274(15):10618-10624 (1999). Gong, L., et al., FEBS Lett. 448(1):185-189 (1999). Okuma, T., et al., Biochem. Biophys. Res. Commun. 254(3):693-698 (1999).
