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MRPS12 Antibody (Center K43)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | O15235 |
| Other Accession | NP_203526.1, NP_066930.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 15173 Da |
| Antigen Region | 28-59 aa |
| Gene ID | 6183 |
|---|---|
| Other Names | 28S ribosomal protein S12, mitochondrial, MRP-S12, S12mt, MT-RPS12, MRPS12, RPMS12, RPSM12 |
| Target/Specificity | This MRPS12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-59 amino acids from the Central region of human MRPS12. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MRPS12 Antibody (Center K43) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MRPS12 |
|---|---|
| Synonyms | RPMS12, RPSM12 |
| Cellular Location | Mitochondrion. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein.
References
Zanotto, E., et al. Biochim. Biophys. Acta 1789(5):432-442(2009)
Russo, A., et al. Biochim. Biophys. Acta 1779(12):820-829(2008)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Cui, Y.P., et al. World J. Gastroenterol. 9(9):1892-1896(2003)
Zhang, Z., et al. Genomics 81(5):468-480(2003)
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