- CITATIONS: 3
|Application ||WB, IHC-P, FC, E|
|Other Accession||Q9R0E2, NP_000293.2|
|Calculated MW||83550 Da|
|Antigen Region||66-94 aa|
|Other Names||Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1, Lysyl hydroxylase 1, LH1, PLOD1, LLH, PLOD|
|Target/Specificity||This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PLOD1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PLOD1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.|
|Cellular Location||Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side|
Provided below are standard protocols that you may find useful for product applications.
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Huang, Q.Y., et al. Bone 44(5):984-988(2009)
Yamada, Y., et al. Int. J. Mol. Med. 19(5):791-801(2007)
Tasker, P.N., et al. Osteoporos Int 17(7):1078-1085(2006)
Giunta, C., et al. Mol. Genet. Metab. 86 (1-2), 269-276 (2005) :
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.