|Application ||WB, FC, E|
|Other Accession||NP_055883.2, NP_001035242.1, NP_001171891.1, NP_001171890.1|
|Calculated MW||80173 Da|
|Antigen Region||1-30 aa|
|Other Names||Retrotransposon-derived protein PEG10, Embryonal carcinoma differentiation-regulated protein, Mammalian retrotransposon-derived protein 2, Myelin expression factor 3-like protein 1, MEF3-like protein 1, Paternally expressed gene 10 protein, Retrotransposon gag domain-containing protein 3, Retrotransposon-derived gag-like polyprotein, Ty3/Gypsy-like protein, PEG10, EDR, KIAA1051, MAR2, MART2, MEF3L1, RGAG3|
|Target/Specificity||This PEG10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PEG10.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PEG10 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EDR, KIAA1051, MAR2, MART2, MEF3L1, RGAG|
|Function||Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter (By similarity).|
|Cellular Location||Nucleus. Cytoplasm. Note=Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells and in the Hep-G2 cell line. Colocalized with ALK1|
|Tissue Location||Expressed in the cytotrophoblast layer but not in the overlying syncytiotrophoblast of the placenta. Expressed in prostate and breast carcinomas but not in normal breast and prostate epithelial cells. Expressed in the Hep-G2 cell line (at protein level). Expressed in brain, liver, spleen, kidney, thymus, lung, ovary, testis, reactive lymph node, skeletal muscle, adipose tissue and placenta. Expressed in pancreatic and hepatocellular carcinomas (HCC).|
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Provided below are standard protocols that you may find useful for product applications.
This is a paternally expressed imprinted gene that encodes transcripts containing two overlapping open reading frames (ORFs), RF1 and RF1/RF2, as well as retroviral-like slippage and pseudoknot elements, which can induce a -1 nucleotide frame-shift. ORF1 encodes a shorter isoform with a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons. The longer isoform is the result of -1 translational frame-shifting leading to translation of a gag/pol-like protein combining RF1 and RF2. It contains the active-site consensus sequence of the protease domain of pol proteins. Additional isoforms resulting from alternatively spliced transcript variants, as well as from use of upstream non-AUG (CUG) start codon, have been reported for this gene. Increased expression of this gene is associated with hepatocellular carcinomas.
Tsuji, K., et al. Cancer Genet. Cytogenet. 198(2):118-125(2010)
Chang, Y., et al. Zhonghua Gan Zang Bing Za Zhi 18(4):288-291(2010)
Lux, H., et al. PLoS ONE 5 (1), E8686 (2010) :
Wang, C., et al. FEBS Lett. 582(18):2793-2798(2008)
Lux, A., et al. J. Biol. Chem. 280(9):8482-8493(2005)
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