|Application ||WB, E|
|Other Accession||Q6GQ95, A0JPN6, Q62276, NP_598395.1, NP_852468.1|
|Predicted||Mouse, Rat, Xenopus|
|Calculated MW||22221 Da|
|Antigen Region||1-30 aa|
|Other Names||Mediator of RNA polymerase II transcription subunit 22, Mediator complex subunit 22, Surfeit locus protein 5, Surf-5, MED22, SURF5|
|Target/Specificity||This MED22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MED22.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MED22 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.|
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Provided below are standard protocols that you may find useful for product applications.
This gene is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Hillman, R.T., et al. Genome Biol. 5 (2), R8 (2004) :
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