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MED22 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q15528 |
| Other Accession | Q6GQ95, A0JPN6, Q62276, NP_598395.1, NP_852468.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 22221 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 6837 |
|---|---|
| Other Names | Mediator of RNA polymerase II transcription subunit 22, Mediator complex subunit 22, Surfeit locus protein 5, Surf-5, MED22, SURF5 |
| Target/Specificity | This MED22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MED22. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MED22 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MED22 |
|---|---|
| Synonyms | SURF5 |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
| Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Hillman, R.T., et al. Genome Biol. 5 (2), R8 (2004) :
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