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SKA2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SKA2 Antibody (N-term) AP12680a
    SKA2 Antibody (N-term) (Cat. #AP12680a) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SKA2 antibody detected the SKA2 protein (arrow).
    detail
  • IHC-P - SKA2 Antibody (N-term) AP12680a
    SKA2 Antibody (N-term) (Cat. #AP12680a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SKA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q8WVK7
Other Accession Q4R8E8, NP_872426.1
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 14188 Da
Antigen Region 9-37 aa
Additional Information
Gene ID 348235
Other Names Spindle and kinetochore-associated protein 2, Protein FAM33A, SKA2, FAM33A
Target/Specificity This SKA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human SKA2.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSKA2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SKA2
Synonyms FAM33A
Function Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization- coupled manner (PubMed:17093495, PubMed:19289083). In the complex, it is required for SKA1 localization (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020).
Cellular Location Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore- associated proteins.
Research Areas
Citations (0)
citation

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Background

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization.

References

Cao, G., et al. Biochem. Biophys. Res. Commun. 396(4):978-982(2010)
Welburn, J.P., et al. Dev. Cell 16(3):374-385(2009)
Rice, L., et al. J. Endocrinol. 198(3):499-509(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Hanisch, A., et al. EMBO J. 25(23):5504-5515(2006)

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$ 365.00
$ 140.00
Cat# AP12680a
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