|Application ||WB, IHC-P, E|
|Other Accession||Q4R8E8, NP_872426.1|
|Calculated MW||14188 Da|
|Antigen Region||9-37 aa|
|Other Names||Spindle and kinetochore-associated protein 2, Protein FAM33A, SKA2, FAM33A|
|Target/Specificity||This SKA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human SKA2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SKA2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:17093495, PubMed:19289083). In the complex, it is required for SKA1 localization (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020).|
|Cellular Location||Cytoplasm, cytoskeleton, spindle Chromosome, centromere, kinetochore. Note=Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins.|
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Provided below are standard protocols that you may find useful for product applications.
Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization.
Cao, G., et al. Biochem. Biophys. Res. Commun. 396(4):978-982(2010)
Welburn, J.P., et al. Dev. Cell 16(3):374-385(2009)
Rice, L., et al. J. Endocrinol. 198(3):499-509(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Hanisch, A., et al. EMBO J. 25(23):5504-5515(2006)
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