|Application ||WB, E|
|Other Accession||Q921M3, A0JN52, NP_036558.3|
|Calculated MW||135577 Da|
|Antigen Region||1046-1074 aa|
|Other Names||Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SF3b130, STAF130, Spliceosome-associated protein 130, SAP 130, SF3B3, KIAA0017, SAP130|
|Target/Specificity||This SF3B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1046-1074 amino acids from the C-terminal region of human SF3B3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SF3B3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Will, C.L., et al. RNA 10(6):929-941(2004)
Cavusoglu, N., et al. Proteomics 3(2):217-223(2003)
Will, C.L., et al. EMBO J. 21(18):4978-4988(2002)
Martinez, E., et al. Mol. Cell. Biol. 21(20):6782-6795(2001)
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