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SF3B3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SF3B3 Antibody (C-term) AP12703b
    All lanes : Anti-SF3B3 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SF3B3 Antibody (C-term) AP12703b
    SF3B3 Antibody (C-term) (Cat. #AP12703b) western blot analysis in MDA-MB453,Jurkat cell line lysates (35ug/lane).This demonstrates the SF3B3 antibody detected the SF3B3 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15393
Other Accession Q921M3, A0JN52, NP_036558.3
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 135577 Da
Antigen Region 1046-1074 aa
Additional Information
Gene ID 23450
Other Names Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SF3b130, STAF130, Spliceosome-associated protein 130, SAP 130, SF3B3, KIAA0017, SAP130
Target/Specificity This SF3B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1046-1074 amino acids from the C-terminal region of human SF3B3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSF3B3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SF3B3
Synonyms KIAA0017, SAP130
Function Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Cellular Location Nucleus.
Citations (0)

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This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Will, C.L., et al. RNA 10(6):929-941(2004)
Cavusoglu, N., et al. Proteomics 3(2):217-223(2003)
Will, C.L., et al. EMBO J. 21(18):4978-4988(2002)
Martinez, E., et al. Mol. Cell. Biol. 21(20):6782-6795(2001)

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$ 295.00
$ 99.00
Cat# AP12703b
(40 western blots)
Availability: In Stock
Bulk Size
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