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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   KRT12 Antibody (C-term)   

KRT12 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - KRT12 Antibody (C-term) AP12735b
    Overlay histogram showing K562 cells stained with AP12735b (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP12735b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - KRT12 Antibody (C-term) AP12735b
    Anti-KRT12 Antibody (C-term) at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - KRT12 Antibody (C-term) AP12735b
    Anti-KRT12 Antibody (C-term)at 1:2000 dilution + mouse eyeball lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - KRT12 Antibody (C-term) AP12735b
    Anti-KRT12 Antibody (C-term)at 1:500 dilution + Y79 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - KRT12 Antibody (C-term) AP12735b
    KRT12 Antibody (C-term) (Cat. #AP12735b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the KRT12 antibody detected the KRT12 protein (arrow).
  • IHC-P - KRT12 Antibody (C-term) AP12735b
    KRT12 Antibdy (C-term) (Cat. #AP12735b)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT12 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q99456
Other Accession NP_000214.1
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 53511 Da
Antigen Region 442-471 aa
Additional Information
Gene ID 3859
Other Names Keratin, type I cytoskeletal 12, Cytokeratin-12, CK-12, Keratin-12, K12, KRT12
Target/Specificity This KRT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the C-terminal region of human KRT12.
Dilution FC~~1:25
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKRT12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT12
Function May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity).
Tissue Location Cornea specific..
Research Areas
Citations ( 0 )

Background

KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.

References

Clausen, I., et al. Mol. Vis. 16, 954-960 (2010) :
Seto, T., et al. Jpn. J. Ophthalmol. 52(3):224-226(2008)
Nielsen, K., et al. Cornea 27(1):100-102(2008)
Sullivan, L.S., et al. Mol. Vis. 13, 975-980 (2007) :
Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)

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$ 295.00
$ 99.00
Cat# AP12735b
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(40 western blots)
Availability: In Stock
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