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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   POFUT1 Antibody (C-term)   

POFUT1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - POFUT1 Antibody (C-term) AP12747b
    POFUT1 Antibody (C-term) (Cat. #AP12747b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the POFUT1 antibody detected the POFUT1 protein (arrow).
  • IHC-P - POFUT1 Antibody (C-term) AP12747b
    POFUT1 Antibody (C-term) (Cat. #AP12747b)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POFUT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H488
Other Accession NP_056167.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43956 Da
Antigen Region 344-373 aa
Additional Information
Gene ID 23509
Other Names GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1, O-FucT-1, POFUT1, FUT12, KIAA0180
Target/Specificity This POFUT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-373 amino acids from the C-terminal region of human POFUT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOFUT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms FUT12, KIAA0180
Function Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).
Cellular Location Endoplasmic reticulum.
Tissue Location Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Research Areas
Citations (0)

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This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq].


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Stahl, M., et al. J. Biol. Chem. 283(20):13638-13651(2008)
Luo, Y., et al. J. Biol. Chem. 280(12):11289-11294(2005)
Shi, S., et al. Proc. Natl. Acad. Sci. U.S.A. 100(9):5234-5239(2003)
Panin, V.M., et al. J. Biol. Chem. 277(33):29945-29952(2002)

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$ 295.00
$ 99.00
Cat# AP12747b
(40 western blots)
Availability: In Stock
Bulk Size
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