|Application ||WB, IHC-P, E|
|Calculated MW||43956 Da|
|Antigen Region||344-373 aa|
|Other Names||GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1, O-FucT-1, POFUT1, FUT12, KIAA0180|
|Target/Specificity||This POFUT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-373 amino acids from the C-terminal region of human POFUT1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||POFUT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).|
|Cellular Location||Endoplasmic reticulum.|
|Tissue Location||Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq].
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Stahl, M., et al. J. Biol. Chem. 283(20):13638-13651(2008)
Luo, Y., et al. J. Biol. Chem. 280(12):11289-11294(2005)
Shi, S., et al. Proc. Natl. Acad. Sci. U.S.A. 100(9):5234-5239(2003)
Panin, V.M., et al. J. Biol. Chem. 277(33):29945-29952(2002)
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