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WHSC1L1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - WHSC1L1 Antibody (N-term) AP12757a
    Western blot analysis of WHSC1L1 (arrow) using rabbit polyclonal WHSC1L1 Antibody (N-term) (Cat. #AP12757a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the WHSC1L1 gene.
    detail
  • WB - WHSC1L1 Antibody (N-term) AP12757a
    Anti-WHSC1L1 Antibody (N-term)at 1:2000 dilution + mouse brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 162 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9BZ95
Other Accession NP_060248.2, NP_075447.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 161613 Da
Antigen Region 188-217 aa
Additional Information
Gene ID 54904
Other Names Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, Wolf-Hirschhorn syndrome candidate 1-like protein 1, WHSC1-like protein 1, WHSC1L1, NSD3
Target/Specificity This WHSC1L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the N-terminal region of human WHSC1L1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWHSC1L1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NSD3 (HGNC:12767)
Synonyms WHSC1L1
Function Histone methyltransferase. Preferentially dimethylates 'Lys- 4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
Cellular Location Nucleus. Chromosome.
Tissue Location Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung
Citations (0)
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Background

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described.

References

Zhou, Z., et al. Biochem. Biophys. Res. Commun. 398(3):565-570(2010)
Kim, S.M., et al. Biochem. Biophys. Res. Commun. 345(1):318-323(2006)
Tonon, G., et al. Proc. Natl. Acad. Sci. U.S.A. 102(27):9625-9630(2005)
Rosati, R., et al. Blood 99(10):3857-3860(2002)
Stec, I., et al. Genomics 76 (1-3), 5-8 (2001) :

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$ 182.50
$ 70.00
Cat# AP12757a
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