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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CDCA8 Antibody (N-term)   

CDCA8 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CDCA8 Antibody (N-term) AP12760a
    Western blot analysis of CDCA8 (arrow) using rabbit polyclonal CDCA8 Antibody (N-term) (Cat. #AP12760a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDCA8 gene.
  • IHC-P - CDCA8 Antibody (N-term) AP12760a
    CDCA8 Antibody (N-term) (Cat. #AP12760a)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDCA8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - CDCA8 Antibody (N-term) AP12760a
    CDCA8 Antibody (N-term) (Cat. #AP12760a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q53HL2
Other Accession NP_060571.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31323 Da
Antigen Region 9-38 aa
Additional Information
Gene ID 55143
Other Names Borealin, Cell division cycle-associated protein 8, Dasra-B, hDasra-B, Pluripotent embryonic stem cell-related gene 3 protein, CDCA8, PESCRG3
Target/Specificity This CDCA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human CDCA8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDCA8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDCA8
Synonyms PESCRG3
Function Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
Cellular Location Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells
Research Areas
Citations (0)

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CDCA8 is a component of a chromosomal passenger complex required for stability of the bipolar mitotic spindle (Gassmann et al., 2004 [PubMed 15249581]).


Bourhis, E., et al. Biochemistry 48(29):6783-6793(2009)
Zhang, Q., et al. Mol. Biol. Rep. 36(3):603-609(2009)
Klein, U.R., et al. Mol. Biol. Cell 20(1):410-418(2009)
Xia, F., et al. Mol. Cell. Biol. 28(17):5299-5311(2008)
Jelluma, N., et al. Cell 132(2):233-246(2008)

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Cat# AP12760a
(40 western blots)
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