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ELF4 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ELF4 Antibody (N-term) AP12761A
    Western blot analysis of ELF4 (arrow) using rabbit polyclonal ELF4 Antibody (N-term) (Cat. #AP12761a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ELF4 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99607
Other Accession NP_001412.1, NP_001120669.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 70730 Da
Antigen Region 161-188 aa
Additional Information
Gene ID 2000
Other Names ETS-related transcription factor Elf-4, E74-like factor 4, Myeloid Elf-1-like factor, ELF4 {ECO:0000312|EMBL:CAI428821}
Target/Specificity This ELF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-188 amino acids from the N-terminal region of human ELF4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsELF4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ELF4 {ECO:0000312|EMBL:CAI42882.1}
Function Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2 (By similarity). Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway.
Cellular Location Nucleus, PML body. Note=Accumulation into PML nuclear bodies is mediated by PML
Tissue Location Abundantly expressed in the placenta and in a variety of myeloid leukemia cell lines. Moderate levels of expression in heart, lung, spleen, thymus, peripheral blood lymphocytes, ovary and colon. Lower levels of expression in Jurkat T-cells and other T-cell lines and no expression in brain
Citations (0)

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The protein encoded by this gene is a transcriptional activator that binds and activates the promoters of the CSF2, IL3, IL8, and PRF1 genes. The encoded protein is involved in natural killer cell development and function, innate immunity, and induction of cell cycle arrest in naive CD8+ cells. Two transcript variants encoding the same protein have been found for this gene.


Yamada, T., et al. Nat. Immunol. 10(6):618-626(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Moore, S.D., et al. Leuk. Res. 30(8):1037-1042(2006)
Liu, Y., et al. Mol. Cell. Biol. 26(8):3114-3123(2006)
Koga, T., et al. FEBS Lett. 579(13):2811-2816(2005)

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Cat# AP12761A
(40 western blots)
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