|Application ||FC, WB, IHC-P, E|
|Calculated MW||44647 Da|
|Antigen Region||221-249 aa|
|Other Names||Speckle-type POZ protein-like, HIB homolog 2, Roadkill homolog 2, SPOPL|
|Target/Specificity||This SPOPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-249 amino acids from the Central region of human SPOPL.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPOPL Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, but with relatively low efficiency. Cullin-RING-based BCR (BTB-CUL3- RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL are less efficient than ubiquitin ligase complexes containing only SPOP. May function to down-regulate the activity of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain SPOP.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
In complex with a cullin, may act in ubiquitination and proteasomal degradation processes (By similarity).
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Zhang, Q., et al. Dev. Cell 10(6):719-729(2006)
Kent, D., et al. Development 133(10):2001-2010(2006)
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