|Application ||FC, WB, IHC-P, E|
|Calculated MW||44647 Da|
|Antigen Region||221-249 aa|
|Other Names||Speckle-type POZ protein-like, HIB homolog 2, Roadkill homolog 2, SPOPL|
|Target/Specificity||This SPOPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-249 amino acids from the Central region of human SPOPL.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPOPL Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, but with relatively low efficiency. Cullin-RING-based BCR (BTB-CUL3- RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL are less efficient than ubiquitin ligase complexes containing only SPOP. May function to down-regulate the activity of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain SPOP.|
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Provided below are standard protocols that you may find useful for product applications.
In complex with a cullin, may act in ubiquitination and proteasomal degradation processes (By similarity).
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Zhang, Q., et al. Dev. Cell 10(6):719-729(2006)
Kent, D., et al. Development 133(10):2001-2010(2006)
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